Physicians who are interested in furthering their patient treatment options, our PRP training courses and other biologic courses offer hands on training to fully engage the student.  The Regenerative Medicine Training Institute offers a variety of courses to teach prp protocols and platelet rich plasma procedure techniques.

Dr. Joseph Ruane lectures at RMTI

Dr. Joseph Ruane lectures at RMTI

What are platelets..

Platelets are the cells that circulate within our blood and bind together when they recognize damaged blood vessels.  

Platelets, the smallest of our blood cells, can only be seen under a microscope. They’re literally shaped like small plates in their non-active state.  A blood vessel will send out a signal when it becomes damaged. When platelets receive that signal, they’ll respond by traveling to the area and transforming into their “active” formation.

A normal platelet count ranges from 150,000 to 450,000 platelets per microliter of blood.  

What are cytokines...

Cytokines are cell signaling molecules that aid cell to cell  communication in immune responses and stimulate the movement of cells towards sites of inflammation, infection and trauma.

Are “immunomodulation agents" or agents that modulate or alter the  immune system response.

Cytokines are important regulators of both the innate and adaptive  immune response.

What is Platelet Rich Plasma...

Sometimes referred to as autologous Platelet Rich Plasma (meaning using patient’s own blood). Blood typically contains 6% platelets whereas PRP has a significantly increased supra-physiological platelet concentration.

Platelet Rich Plasma is a small volume of plasma, in which proteins, cytokines, red –and white blood cells, and concentrated platelet are suspended (4-7 times, when compared to native levels). Platelet level can vary depending on the method of extraction and equipment, studies have shown that clinical benefit can be obtained if the PRP used has an increased platelet concentration of 4x greater than normal blood

Normal blood contains around 150,000 to 450,000 platelets/microliter, therapeutic PRP should contain 1.0million platelet/microliter.

When applied to a tissue, or injury sites, it induces tissue regeneration through multiple pathways, like platelet growth factor release and cell mediating cytokines.

Platelet must be activated at the level of tissue injury in order for the PRP graft to be successful.  During activation the platelet successfully releases their content and begins the healing cascade of events that leads to the restoration and growth of normal collagen.  Wound healing or collagen repair can be separated into three separate phases or stages (inflammation, proliferation and remodeling).

You can learn more about Platelet rich plasma training in one of our courses here!

How does PRP work...

In our PRP Courses, RMTI teaches students the importance of how PRP works. Platelets contain many growth factors, at least 5-6 are well known and have previously been proven to be vital to bone and soft tissue healing.

1. High concentration of platelets yielding various healing growth factors:

  • PDGF (Platelet derived growth factor)
  • TGF-α&β (Transforming Growth alpha and beta)
  • EFG-(Epidermal growth factor)
  • FGF-(Fibroblast growth factor)
  • IGF-(insulin Growth Factor)
  • PDAF-(Platelet derived angiogenesis factor)

2. High concentration of WBC and Phagocytic cells

3. Vasoactive and chemotactic agents


Jeremy Carreno MA performs a perfect 60cc blood draw.

Platelet Rich Plasma Training starts with the Blood Draw

For all PRP and Platelet Rich Plasma procedures, blood is required.  Depending on the type of injury or location of the injury, the required whole blood amount could vary.  It is important to have a final product that optimally provides the quickest and most complete healing cascade.  Processing of PRP into either leukocyte poor or leukocyte rich PRP is mandatory by many doctors.  Other white blood cells and components of blood have different healing qualities.  As we learn more about these protocols and results, RMTI's PRP courses will continue to evolve with the information we collect.

- content on this page include excerpts from RMTI Lecturers Marcia James and Jeremy Carreno